Composite
Part:BBa_K208045:Design
Designed by: USU iGEM 2009 Group: iGEM09_Utah_State (2009-10-20)
Lac Promoter/RBS/GFP/Term
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 230
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 749
Illegal XhoI site found at 650 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lac promoter, RBS, GFP, and Terminator BioBricks were ligated together to make this composite part. This part is in plasmid pSB1AK3
Source
This part was constructed by performing several step-wise ligations of parts that were either already present in the registry (BBa_B0015) or recently added (as of 2009).
References
Please see the original pages for these Biobricks for more details on references and Silver-fusion.